ELF webinar: Guidelines for High Throughput Screening assay development

10 February 2021

How to optimise your assay for High Throughput Screening (HTS) at the European Lead Factory (ELF). This was the topic of the latest ELF webinar, which took place on Thursday 28 January.

Our guest speaker for the session was Dr Saman Honarnejad, Director of Drug Discovery at Pivot Park Screening Centre (PPSC), the ELF partner responsible for HTS assay development and Ultra HTS campaigns. Together with the other ELF partners, PPSC helps researchers in their hunt for new leads for drug development.

Saman’s presentation provided guidelines for HTS assay development, with an emphasis on the methods to design, evaluate and improve conditions for biochemical and cellular assay formats. Having an assay that is compatible with the ELF screening systems increases ones chances for a screening slot at the ELF and is essential to the success of a screening campaign.

Key take home messages from the session include:

  • Know your target well (acquire all the information available in the literature on your target) and ask the right questions when it comes to selecting the right assay and optimal conditions
  • Make sure you have sound biochemistry (kinetics (Km/Kd), linearity, etc.)
  • Use robust statistics to evaluate assay parameters and performance
  • And perhaps most importantly, get in touch with the ELF programme office to help you connect to the ELF expert during assay development

The 1-hour webinar attracted a good turn-out of close to 70 participants. If you were not able to attend, a recording of Saman’s presentation is available to view online.

The next ELF webinar will take place on Thursday 11 March from 11:00-12:00 (CET) and will focus on High Content Screening with a presentation from guest speaker, Professor Jason Swedlow, head of the National Phenotypic Screening Centre at the University of Dundee – one of the European Lead Factory’s academic partners. Attendance is free, but registration is required via this link.