ELF use of the “hot” technique MST

20 February 2018

The European Lead Factory (ELF) scientists are not shy of applying new techniques to deliver the best possible results. A recent perspective, published in SLAS Discovery, describes how getting hot (or not) can help in the triaging of primary HTS hits.

The biophysical technique Microscale Thermophoresis (MST) measures differences in the rate and direction of movement of biomolecules through a temperature gradient caused when binding in complex with small molecules. The ELF experts employ this, alongside a range of other biophysical and biochemical assays during an HTS campaign to identify the most promising hits. Biophysical methods, such as MST, are now integral to the latter stages of HTS triage at the ELF. ‘This is a key step to avoid wasting resources following up compounds with unproductive/non-specific mechanisms of action.’ Stuart McElroy, leading the hit characterisation team at European Screening Centre Newhouse, explained.

The article gives an overview of the MST assay development workflow used at the ELF and a perspective of our experience to date of using MST to triage the output of HTS campaigns, comparing and contrasting it with other biophysical techniques.